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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial role in endorsing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants have been documented but comprehensive nucleotide sequence data is not really but offered.
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Blocking peptides are peptides that bind exclusively into the goal antibody and block antibody binding. These peptide generally is made up of the epitope regarded via the antibody.
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This tutorial outlines more attainable optimization of cellular and immunoassay parameters to be sure the absolute best benefits are...
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This manual outlines even more possible optimization of mobile and immunoassay parameters to ensure the best possible results are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining in the blocked antibody compared to the antibody on your own, one can see which staining is certain.
Focus on Info Cyclin E belongs to your highly conserved cyclin household, whose members are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins functionality as regulators of CDK kinases. Various cyclins exhibit unique expression and degradation designs which add into the temporal coordination of every mitotic party. Cyclin E varieties a complex with and features as being a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S transition. This protein accumulates at the G1-S section boundary which is degraded as cells development by way of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by each antibodies...
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